University of Illinois
71 RAL, Box 63-5
600 South Mathews Avenue
Urbana, IL 61801
Additional Campus Affiliations
Professor, Carl R. Woese Institute for Genomic Biology
Professor, Beckman Institute for Advanced Science and Technology
Director, School of Chemical Sciences
Department Affiliate, Molecular and Integrative Physiology
CAS Professor, Center for Advanced Study
Professor, Micro and Nanotechnology Lab
Professor, Carle Illinois College of Medicine
Affiliate, Neuroscience Program
Professor Sweedler received his B.S. degree in Chemistry from the University of California at Davis in 1983, and his Ph.D. from the University of Arizona in 1989. Thereafter, he was an NSF Postdoctoral Fellow at Stanford University before joining the faculty at Illinois in 1991. His research interests are in bioanalytical chemistry, and focus on developing new methods for assaying the chemistry occurring in nanoliter-volume samples, and applying these analytical methods to characterize the molecular forms, distribution, and dynamic release of neurotransmitters and neuropeptides from a range of animal models. Professor Sweedler is Editor-in-Chief of the journal Analytical Chemistry.
analytical neurochemistry, more specifically, studies on cell to cell signaling pathways involved in learning, memory, and behavior, as well as conserved aspects of neurotransmission across the metazoan; dynamic studies of subcellar neurotransmitter distribution and release, ultra-trace peptide analysis
We develop a variety of analytical measurement methodologies, including microfluidic/nanofluidic sampling, capillary electrophoresis separations, and mass spectrometry characterization. These technologies combine to form metabolomics and peptidomics workflows, with much of our efforts directed toward scaling these methods to nanoliter and smaller volume levels. We are currently developing a range of mass spectrometry imaging approaches that allow thousands of individual cells to be characterized for their neuropeptide content, and a unique capillary electrophoresis approach that allows us to sample the cytoplasm for a selected neuron or glia and characterize its metabolome. Many of these measurement capabilities are unique and not currently available elsewhere.
We use these approaches to study cell-to-cell signaling in the central nervous system to uncover novel neurochemical pathways. Because neurotransmitters and neuromodulators are so well conserved across the entire animal kingdom, we work with a wide variety of animal models, from mollusks to insects to vertebrates. We use new peptidomic and metabolomic approaches—many developed by us—to characterize these signaling molecules in samples ranging from a single cell to entire brain regions.
Why are we interested in these neuromodulatory compounds? Because of the important roles they play in behavior, learning, and memory. Cell-to-cell communication in the brain relies upon a surprising array of molecules, from gaseous molecules (e.g., nitric oxide) to classical transmitters (e.g., glutamate), as well as unexpected molecules (e.g., d-serine), and a range of peptides. We study these to understand how networks of neurons and associated supporting cells such as glia can work together to confer emergent properties that give rise to behavior and memory. Specific queries address what molecules are present in specific cells and networks, and how they change based on network activity, animal behavior, or even on exposure to drugs.
Neuropeptides are perhaps the most diverse category of neuromodulators. Using a suite of mass spectrometry-based approaches, we have characterized the neuropeptides and prohormones in the sea slug, honey bee, urchin, planarian, songbird, and in several mammals. Literally hundreds of new prohormones and even more putative neuropeptides have been discovered, and the bioactivity of several of these novel neuropeptides characterized.
In addition to the research described above, a number of collaborative projects are undertaken through the UIUC Neuroproteomics and Neurometabolomics Center on Cell-Cell Signaling and the Center for Nutrition Learning and Memory.
Awards and Honors
Malcom E. Pruitt Award, Council for Chemical Research
Torbern Bergman Medal from the Swedish Chemical Society
ANACHEM Award, Federation of Analytical and Spectroscopy Societies
The Analytical Chemistry Award, The American Chemical Society
Ralph N. Adams Award, The Pittsburgh Conference
Fellow of the American Chemical Society
Theophilus Redwood Lecturer, Royal Society of Chemistry
Viktor Mutt Prize, International Regulatory Peptide Society
Neumann, E. K., Ellis, J. F., Triplett, A. E., Rubakhin, S. S., & Sweedler, J. V. (2019). Lipid Analysis of 30 000 Individual Rodent Cerebellar Cells Using High-Resolution Mass Spectrometry. Analytical Chemistry, 91(12), 7871-7878. https://doi.org/10.1021/acs.analchem.9b01689
Morales-Soto, N., Dunham, S. J. B., Baig, N. F., Ellis, J. F., Madukoma, C. S., Bohn, P. W., Sweedler, J. V., & Shrout, J. D. (2018). Spatially dependent alkyl quinolone signaling responses to antibiotics in Pseudomonas aeruginosa swarms. Journal of Biological Chemistry, 293(24), 9544-9552. https://doi.org/10.1074/jbc.RA118.002605
Checco, J. W., Zhang, G., Yuan, W. D., Le, Z. W., Jing, J., & Sweedler, J. V. (2019). Aplysia allatotropin-related peptide and its newly identified D-amino acid-containing epimer both activate a receptor and a neuronal target. Journal of Biological Chemistry, 293(43), 16862-16873. https://doi.org/10.1074/jbc.RA118.004367
Jansson, E. T., Comi, T. J., Rubakhin, S. S., & Sweedler, J. V. (2016). Single Cell Peptide Heterogeneity of Rat Islets of Langerhans. ACS chemical biology, 11(9), 2588-2595. https://doi.org/10.1021/acschembio.6b00602
Wang, T. A., Yu, Y. V., Govindaiah, G., Ye, X., Artinian, L., Coleman, T. P., Sweedler, J. V., Cox, C. L., & Gillette, M. U. (2012). Circadian rhythm of redox state regulates excitability in suprachiasmatic nucleus neurons. Science, 337(6096), 839-842. https://doi.org/10.1126/science.1222826
Wu, YF., Toba, E. A. D. L., Dvoretskiy, S., Jung, R., Kim, N., Daniels, L., Romanova, E. V., Drnevich, J., Sweedler, J. V., & Boppart, M. D. (Accepted/In press). Development of a cell‐free strategy to recover aged skeletal muscle after disuse. The Journal of Physiology. https://doi.org/10.1113/JP282867
Xie, Y. R., Castro, D. C., Rubakhin, S. S., Sweedler, J. V., & Lam, F. (2022). Enhancing the Throughput of FT Mass Spectrometry Imaging Using Joint Compressed Sensing and Subspace Modeling. Analytical Chemistry, 94(13), 5335-5343. https://doi.org/10.1021/acs.analchem.1c05279
Zhang, Y., Kim, S., Shi, W., Zhao, Y., Park, I., Brenden, C., Iyer, H., Jha, P., Bashir, R., Sweedler, J. V., & Vlasov, Y. (2022). Droplet-assisted electrospray phase separation using an integrated silicon microfluidic platform. Lab on a chip, 22(1), 40-46. https://doi.org/10.1039/d1lc00758k
Burrows, C. J., Huang, J., Wang, S., Kim, H. J., Meyer, G. J., Schanze, K., Lee, T. R., Lutkenhaus, J. L., Kaplan, D., Jones, C., Bertozzi, C., Kiessling, L., Mulcahy, M. B., Lindsley, C. W., Finn, M. G., Blum, J. D., Kamat, P., Choi, W., Snyder, S., ... Laskin, J. (2021). Confronting Racism in Chemistry Journals. ACS Environmental Science and Technology Water, 1(1), 3-5. https://doi.org/10.1021/acsestwater.0c00034
Castro, D. C., Xie, Y. R., Rubakhin, S. S., Romanova, E. V., & Sweedler, J. V. (2021). Image-guided MALDI mass spectrometry for high-throughput single-organelle characterization. Nature Methods, 18(10), 1233-1238. https://doi.org/10.1038/s41592-021-01277-2